《植物生理学报》 2009, 45(4): 363-366

钙和钙调素对机械刺激诱导的烟草悬浮培养细胞耐热性的调控

李忠光*, 龚明

云南师范大学生命科学学院, 昆明650092

通信作者：李忠光；E-mail: zhongguang_li@163.com；Tel: 0871-5517394

摘　要：

机械刺激(mechanical stimulation, MS)可提高烟草悬浮培养细胞的钙调素(calmodulin, CaM)活性, 诱导烟草悬浮培养细 胞耐热性的形成, 外源 Ca2+ 可加强, 而 Ca2+ 螯合剂 EGTA、质膜 Ca2+ 通道阻塞剂 La3+ 和胞内 Ca2+ 通道阻断剂钌红(RR), 以 及 CaM 拮抗剂氯丙嗪(CPZ)和三氟拉嗪(TFP)则削弱这种耐热性的形成。这些暗示 Ca2+ 和 CaM 参与 MS 诱导的烟草悬浮培 养细胞耐热性形成的调控。

关键词：烟草悬浮培养细胞; 机械刺激; 耐热性; 钙; 钙调素

收稿：2009-01-04   修定：2009-02-05

资助：国家自然科学基金(30460016)、云南省自然科学基金(2006C0030M)和云南省教育厅基金(06Y117B)。

Involvement of Calcium and Calmodulin in the Mechanical Stimulation-induced Heat Tolerance in Tobacco (Nicotiana tabacum L.) Suspension Culture Cells

LI Zhong-Guang*, GONG Ming

School of Life Sciences, Yunnan Normal University, Kunming 650092, China

Corresponding author: LI Zhong-Guang; E-mail: zhongguang_li@163.com; Tel: 0871-5517394

Abstract:

Mechanical stimulation (MS) obviously enhanced calmodulin (CaM) activity in tobacco (Nicotiana tabacum) suspension cells, induced the heat tolerance in tobacco suspension cells. The MS-induced heat tolerance in tobacco suspension cells was strengthened with exogenous Ca²⁺ treatment, but weakened by Ca²⁺chelator EGTA, plasmamembrame-channel blocker La³⁺, intracellular channel blocker ruthenium red (RR) and calmodulin antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively. These results suggested that Ca²⁺ and CaM were involved in the regulation of MS-induced heat tolerance in tobacco suspension cells.

Key words: tobacco suspension cells; mechanical stimulation; heat tolerance; calcium; calmodulin